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  • N3037 - Anti-NBS1 (Nibrin), C-terminal antibody produced in rabbit

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N3037 Sigma-Aldrich

Anti-NBS1 (Nibrin), C-terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression, Antibodies to Transcription Regulators,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 2-4 μg using HeLa cell lysates
  indirect immunofluorescence: 5-10 μg/mL
  microarray: suitable
  western blot: 0.25-0.5 μg/mL using extracts of 293T cells transfected with recombinant NBS1
  western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
species reactivity   human
mol wt   antigen 95 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   O60934
Gene Information   human ... NBN(4683)
mouse ... Nbn(27354)
rat ... Nbn(85482)

Description

General description

Nibrin (NBS1) is made up of 754 amino acids.

Immunogen

synthetic peptide corresponding to amino acids 735-753 of human NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the rat and mouse corresponding sequences in two amino acids.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA. Mutations in the gene encoding NBS1 have been associated with Nijmegen breakage syndrome (NBS).

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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Add to Cart

N3162 Anti-NBS1 (Nibrin) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
N9287 Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse, ~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

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