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  • N3162 - Anti-NBS1 (Nibrin) antibody produced in rabbit

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N3162 Sigma-Aldrich

Anti-NBS1 (Nibrin) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-p95 Protein of the MRE11/RAD50 Complex

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression, Antibodies to Transcription Regulators,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg
  indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton
  microarray: suitable
  western blot: 1-2 μg/mL using MCF7 nuclear extracts
species reactivity   rat, human, canine, chimpanzee
mol wt   antigen mol wt 95 kDa (also recognizes 100 kDa band)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   O60934
Gene Information   human ... NBN(4683)
mouse ... Nbn(27354)
rat ... Nbn(85482)

Description

General description

Nibrin (NBS1) is made up of 754 amino acids.

Immunogen

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

N3037 Anti-NBS1 (Nibrin), C-terminal antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
N9287 Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse, ~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

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