N3518 Sigma-Aldrich

NZ Amine® Broth

Lambda bacteriophage growth medium

  •  NACRES NA.85



General description

NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Features and Benefits

NZ Amine powder provides:
• Standard formulation
• Convenient package sizes
• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid


10 g/L Tryptone
5 g/L NaCl
1 g/L MgSO4


Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

N-Z-Amine is a registered trademark of Kerry Group

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins


Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Peer-Reviewed Papers


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