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N3755 Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym: Endonuclease micrococcal, MNase, Micrococcal endonuclease, Micrococcal nuclease

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   300
form   lyophilized powder
specific activity   100-300 units/mg protein
composition   Protein, ≥40% E1%/280 (Balance primarily sodium citrate)
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C
Gene Information   Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Description

Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Packaging

50, 200, 500 units in serum bottle

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.

Hydrolyzes 5′-phosphodiester bonds of DNA.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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