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N3786 Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Synonym: Acyl-neuraminyl Hydrolase, Neuraminidase from Arthrobacter ureafaciens, Sialidase

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Description

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Quality

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Unit Definition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Physical form

Lyophilized powder

Kit component only

Description

Product #

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Neuraminidase from Arthrobacter ureafaciens 25 U    
Neuraminidase 5X Reaction Buffer 1.5 mL    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QQ3450000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Glycan Sequencing Using Exoglycosidases

Enzymatic analysis of oligosaccharides using highly specific exoglycosidases, either sequentially or in a matrix array, is a powerful technique in determining the sequence and structure of glycans. E...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Chromatography, Degradations, Digestions, Electron capture detector, Electrophoresis, Error, Gel electrophoresis, Glycosylations, Immobilization, Mass spectrometry, Methods, PAGE, Phase transitions, Separation, Sequences, Sequencing, Spectroscopy

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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