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N3787 Sigma-Aldrich

Monoclonal Anti-Netrin-1 antibody produced in mouse

clone 158936, purified immunoglobulin, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
conjugate   unconjugated
clone   158936, monoclonal
biological source   mouse
application(s)   western blot: 1-2 μg/mL using rmNetrin-1
species reactivity   mouse
form   lyophilized powder
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   O09118
Gene Information   mouse ... Ntn1(18208)

Description

General description

Netrin-1 (NTN1) is a secreted protein.

Specificity

This antibody shows 100% cross-reactivity with rchNetrin-1 and no cross-reactivity with rhNetrin-2, rhNetrin-4, rmNetrin-4, or rmNetrin-G1a.

Immunogen

purified, NS0-derived, recombinant mouse Netrin-1.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California. Patent numbers 5,565,331; 6,096,866; 6,017,714; 6,309,638; and 6,670,451.

Biochem/physiol Actions

Netrin-1 (NTN1) acts as a cardioprotectant. It can stimulate neovascularization of the retina in retinopathy. NTN1 has roles in angiogenesis and thymocyte adhesion.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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