N4014 Sigma


powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym: Fungicidin, Mycostatin



Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antifungal,
Quality Level   PREMIUM
sterility   γ-irradiated
product line   BioXtra
form   powder
mol wt   mol wt 926.1 g/mol
packaging   vial of 240,000 units
application(s)   cell culture | mammalian: suitable
solubility   H2O: insoluble
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
storage temp.   −20°C



Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells. This product is recommended for use in cell culture applications at 50 mg/L.

Biochem/physiol Actions

Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol.

Antimicrobial spectrum: Neomycin acts against fungi, yeasts and molds.


Nystatin in tissue culuture media is stable at 37°C for three days. It is also stable in moderately alkaline media. Solutions and aqueous suspensions begin to lose activity soon after preparation, with heat, light, and oxygen accelerating the decomposition. Aqueous suspensions are stable for 10 minutes when heating to 100°C at pH 7.

Preparation Note

Nystatin is soluble at 28°C in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL), and ethylene glycol (8.75 mg/mL). It is also soluble in DMSO at 5 mg/mL and freely soluble in DMF and formamide. It is not recommended to autoclave or sterile filter solutions of Nystatin. Nystatin is also effective as a suspension, stable at 37°C for 3 days. Prepare 50 mg/mL stock suspensions in water and store at -20°C. This product is ?-irradiated from N6261.

General description

Chemical structure: polyene

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Store under inert gas.Air and light sensitive.Keep in a dry place

Protocols & Applications

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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