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N4915 Sigma-Aldrich

Nitric Oxide Synthase Neuronal Human

recombinant, expressed in E. coli

Synonym: NOS, nNOS

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Properties

recombinant   expressed in E. coli
form   buffered aqueous glycerol solution
activity   350-450 units/mg protein
mol wt   160 kDa
shipped in   dry ice
storage temp.   −70°C

Description

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine in nervous tissues. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.

Unit Definition

One unit of enzyme activity represent the amount of enzyme that produce 1nmole of nitric oxide per minute at 37oC in 40mM Tris-Hcl, or Hepes or EPPs buffer ,PH7.5 containg 5-10 uM Oxyhemoglobin. 1mM NADPH, 2uM FMN/FAD, 5uM H4B , 100uM L-arginine, 50units/ml Catalase, 5units/ml SOD, 0.1mg/ml BSA, 35ul of 9mM EDTA and and 35 ul of 150mg/ml CaM and 12.5mM CaCl2

Physical form

40Mm EPS buffer PH7.5 100mMNaCl, 5% glycerol, 5uM H4B, 100uM L-arginine.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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