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  • N5264 - Monoclonal Anti-Neurofilament 160 antibody produced in mouse

N5264 Sigma

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

clone NN18, ascites fluid

Synonym: Monoclonal Anti-Neurofilament medium chain



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
species reactivity   pig, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections
  immunohistochemistry (frozen sections): suitable
  western blot: suitable
clone   NN18, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 160 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
biological source   mouse
conjugate   unconjugated



neurofilaments from pig spinal cord.

General description

Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation. Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.


Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot.

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Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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