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  • N6786 - Anti-Notch1 antibody, Mouse monoclonal

N6786 Sigma-Aldrich

Anti-Notch1 antibody, Mouse monoclonal

clone mN1A, purified from hybridoma cell culture

Synonym: Notch antibody



Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
conjugate   unconjugated
clone   mN1A, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa).
species reactivity   mouse, human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P46531
Gene Information   human ... NOTCH1(4851)
mouse ... Notch1(18128)


General description

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.


Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.


recombinant protein containing the cdc10-NCR region of mouse Notch1.


Monoclonal Anti-Notch1 antibody is suitable for use in ELISA, immunoblotting, and immunocytochemistry applications to study cell development and fate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


200 μL in glass insert

Biochem/physiol Actions

NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles


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Western Blot Protocol | Immunoblotting Protocol

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Peer-Reviewed Papers


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N4788 Anti-NOTCH1 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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