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N7271 Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonym: Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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Description

Packaging

.2 unit in poly bottle

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Preparation Note

Expressed in glycosidase-free hosts.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QQ3450000

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the reaction conditions of the enzyme ?(23) Neuraminidase, Product No. N7271?
One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.
What is the long term storage and shipping condition of ?(23) Neuraminidase, Product No. N7271?
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
For ?(23) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
The optimum pH range for ?(23 neuraminidase is 6.0. Please see the data sheet.
What is the molecular weight of ?(23) Neuraminidase, Product No. N7271?
The molecular weight of this ?(23 Neuraminidase enzyme is approximately 75 kDa.
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Protocols & Articles

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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