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N7658 Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α1 Subunit) antibody produced in rat

~5 mg/mL, clone mAb 61, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Ion Channels,
conjugate   unconjugated
clone   mAb 61, monoclonal
biological source   rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot: suitable
species reactivity   human, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~5 mg/mL
UniProt accession no.   P02708
Gene Information   human ... CHRNA1(1134)
mouse ... Chrna1(11435)

Description

General description

Cholinergic receptor nicotinic α1 subunit (CHRNA1) is the a subunit of nicotinic acetylcholine receptors. The protein is expressed at neuromuscular junctions. The gene encoding it is localized on human chromosome 2q31.1.

Nicotinic acetylcholine receptors (AChR) are members of a gene superfamily of ligand-gated ion channels, which includes the homologuous γ-aminobutyric acid type A (GABAA) receptors, glycine receptors and 5-HT3 serotonin receptors, but not the structurally dissimilar ligand-gated ion channels comprising the glutamate or ATP receptors. It is likely that all receptors in the AChR superfamily are comprised of five homologous subunits oriented around a central ion channel.

Specificity

Recognizes an epitope with the sequence 371-386 of the α1 subunit of muscle nicotinic acetylcholine receptor. Binds to native and denatured α1 subunit of the nicotinic acetylcholine receptor.

Immunogen

denatured nicotinic acetylcholine receptors from the electric eel Electrophorus.

Application

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α1 Subunit) antibody produced in rat has been used in:
• antibody microarray
• radioimmunoassay (RIA)
• immunohistochemistry
• immunoblotting

Physical form

Solution in 10 mM phosphate buffered saline containing 10 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sold with the permission of the Salk Institute.

Biochem/physiol Actions

Cholinergic receptor nicotinic α1 subunit (CHRNA1) has a role in channel gating. Mutations in the gene have been associated with congenital myasthenic syndromes (CMS).

Nicotinic acetylcholine receptors (AChR) were first characterized in the skeletal muscles and their structural properties were initially characterized in using AChR′s from the homologous electric organ tissue of the Torpedo rays. The functional and structural characterization of neuronal AChR′s developed later due to their lower concentrations in more heterogenous tissues. AChR′s are now being associated with a growing number of diseases.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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