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N8130 Sigma-Aldrich

4-Nitrophenyl acetate

esterase substrate

Synonym: Acetic acid 4-nitrophenyl ester

  • CAS Number 830-03-5

  • Linear Formula CH3CO2C6H4NO2

  • Molecular Weight 181.15

  •  Beilstein/REAXYS Number 515874

  •  EC Number 212-593-5

  •  MDL number MFCD00007326

  •  PubChem Substance ID 24897853

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Chromogen, Enzyme Substrate, Fatty Acyls, Lipids More...
Quality Level   200
assay   ≥98% (TLC)
form   powder or crystals
mp   75-77 °C (lit.)
storage temp.   −20°C
SMILES string   CC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI   1S/C8H7NO4/c1-6(10)13-8-4-2-7(3-5-8)9(11)12/h2-5H,1H3
InChI key   QAUUDNIGJSLPSX-UHFFFAOYSA-N
Gene Information   human ... PON1(5444)

Description

Application

4-Nitrophenyl acetate has been used:
• to evaluate esterase activity in human embryonic kidney cell line
• in carbonic anhydrase inhibition assay
• to study its conversion to p-nitrophenol (p-NP) by the esterase activity of bovine serum albumin
• as a substrate to determine carboxylesterase (CES) activity in mouse liver cells
• to study the advantages of bioconjugation of cross-linked enzyme aggregates (CLEAs) and magnetic iron oxide nanoparticles (MIONPs) in magnetic CLEAs matrix

Packaging

5, 10, 25, 100 g in poly bottle

Substrates

Chromogenic esterase substrate.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
AJ1150000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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