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  • N8158 - Monoclonal Anti-Nicotinic Acetylcholine Receptor (α7 Subunit) antibody produced in rat

N8158 Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α7 Subunit) antibody produced in rat

~5 mg/mL, clone mAb 319, purified immunoglobulin, buffered aqueous solution



conjugate   unconjugated
clone   mAb 319, monoclonal
biological source   rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot: suitable
species reactivity   human, rat, chicken
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
concentration   ~5 mg/mL
Gene Information   chicken ... CHRNA7(374001)
human ... CHRNA7(1139)
rat ... Chrna7(25302)



The antibody recognizes an epitope with amino acids 365-384 of the α7 subunit. Reacts well with chick, but is less reactive with mammalian.


bacterially expressed α7 subunit of the nicotinic acetylcholine receptors.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 10 mM phosphate buffered saline, containing 10 mM sodium azide.

Legal Information

Sold with the permission of the Salk Institute

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers


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