• USA Home
  • N8264 - Nucleoside Phosphorylase from microorganisms

N8264 Sigma-Aldrich

Nucleoside Phosphorylase from microorganisms

lyophilized powder, ≥10 units/mg protein

Synonym: Nucleoside Phosphorylase bacterial, PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase



Related Categories 2.4.x.x Glycosyltransferases, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Chemical Synthesis,
Quality Level   200
form   lyophilized powder
mol wt   ~120 kDa
composition   Protein, 40-80%
storage temp.   −20°C
InChI   1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1


General description

Nucleoside Phosphorylase are trimeric and have a molecular mass of about 100 kDa. Each monomer consists of a mixed β-sheet core flanked by eight α-helices and a purine binding in hydrophobic pocket.

Nucleoside phosphorylase is an enzyme important for the biosynthesis of nucleosides.


Nucleoside Phosphorylase from microorganisms has been used:
•  in phosphopentomutase assay for adenosine synthesis
•  for the conversion of 7-methylthioguanosine to 7-methylthioguanine
•  in the synthesis of cytokinins in lyophilized cotton plant samples

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.


100, 250 units in glass bottle

500, 1000 units in poly bottle

Biochem/physiol Actions

Nucleoside Phosphorylase catalyzes the synthesis of nucleoside derivatives.

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase (XTO-212) and uricase (UAO-201, UAO-211). Purine nucleoside phosophorylase has shown the ability to perform both phosphorylosis and synthesis of purine deoxy- and ribonucleosides. It has also been found that membrane-associated nucleoside phosphorylases may have a transmembranal orientation with their base and ribose-1-P binding sites on opposite sides of the membrane.

Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Physical properties

Isoelectric point : 4.1 +/- 0.1
Michaelis constants : 6.4 x 10-5M (Inosine), 3.2x10-4M (Pi)
Inhibitors : p-Chloromercuribenzoate, SDS, Hg++, Ag+Optimum pH : 7.5 - 8.0
Optimum temperature : 65oC
pH Stability : pH 6.0 - 9.0 (30oC, 16hr)
Thermal stability : below 60oC (pH 7.7, 30min)

Unit Definition

One unit will cause the phosphorolysis of 1.0 μmole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.4 at 25 °C.

Physical form

Lyophilized powder containing potassium gluconate, mannitol and EDTA

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Assay Procedure for Nucleoside Phosphorylase Bacterial

One unit causes the formation of one micromole of uric acid per minute under the conditions described below.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?