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  • N8523 - Monoclonal Anti-NF-κB antibody produced in mouse

N8523 Sigma

Monoclonal Anti-NF-κB antibody produced in mouse

clone NF-12, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
species reactivity   mouse, human
application(s)   immunocytochemistry: suitable
  microarray: suitable
  western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line
clone   NF-12, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 65 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NFKB1(4790)
mouse ... Nfkb1(18033)
biological source   mouse
conjugate   unconjugated



recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).

General description

Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.


Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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