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  • N8539 - Anti-NDRG1 (C-terminal) antibody produced in rabbit

N8539 Sigma

Anti-NDRG1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CAP43, Anti-CMT4D, Anti-DRG1, Anti-GC4, Anti-HMSNL, Anti-N-myc downstream regulated 1, Anti-NDR1 (differentiation-related gene 1 protein), Anti-NMSL, Anti-PROXY1 (protein regulated by oxygen 1), Anti-RIT42, Anti-RTP (reducing agents and tunicamycin-responsive protein), Anti-TARG1, TDD5



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
species reactivity   rat, mouse, human
application(s)   immunoprecipitation (IP): 5-10 μg using lysates of HeLa cells
  western blot: 1-2 μg/mL using whole extract of mouse 3T3 and rat NRK cells
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~43 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NDRG1(10397)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 382-394 of human NDRG1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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WGK Germany  1
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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