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N8630 Sigma-Aldrich

Nuclease P1 from Penicillium citrinum

lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)

Synonym: 3′-Phosphohydrolase, Endonuclease P1, Nuclease 5′-Nucleotidehydrolase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   200
form   lyophilized powder
specific activity   ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
secondary activity   ≥1,000 units/mg protein 3′-nucleotidase
mol wt   42-50 kDa
packaging   vial of ≥250 units (using RNA substrate)
storage temp.   2-8°C

Description

General description

Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.

Application

• Nuclease P1 cleave of single stranded DNA or RNA to 5’ mononucleotides
• Nuclease P1 supports DNA damage and modification research
• Nucleic acids base composition and structural analysis can be done by Nuclease P1
• Nuclease P1 has historically been used for the industrial production of 5′-mononucleotides from yeast RNA.
• Removal of nucleic acids through protein purification can be done by Nuclease P1
• Nuclease P1 is a key reagent for the development of methods for studies involving t-RNA dependent amino acid biosynthesis and t-RNA dependent trans-amidation
• Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent.
• Nuclease P1 was used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.
• Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
The enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 - 8.

The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Biochem/physiol Actions

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

Features and Benefits

Our highly active Nuclease P1 is tested for its 3′- 5′ - Phosphodiesterase Activity and 3′- Nucleotidase Activity and is the most active Nuclease P1 in the market

Physical properties

A zinc dependent glycoprotein consisting of 270 amino acid residues. Molecular mass: 42-50 kDa.

Unit Definition

3′-5′-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Product N8630 (Nuclease P1 from Penicillium citrinum is a lyophilized powder. How should I store solutions of this enzyme?
A solution of Nuclease P1 that has been reconstituted in 20 mM sodium acetate buffer (pH 5.3), 5 mM ZnCl2, and 50 mM sodium chloride can be stored for the short term at 2-8 °C for 2-3 weeks. For longer term storage, at -20 °C, one suggestion is to add 40-50% glycerol after reconstitution in the aqueous buffer. Such solutions (with glycerol added) may be stored for 3-6 months at -20 °C.
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