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N9162 Sigma-Aldrich

Neocarzinostatin from Streptomyces carzinostaticus

≥90% (SDS-PAGE), ~0.5 mg/mL

Synonym: Holoneocarzinostatin, NCS, NSC-69856, Zinostatin



Related Categories Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application, Antibiotics by Mechanism of Action,
Quality Level   200
assay   ≥90% (SDS-PAGE)
concentration   ~0.5 mg/mL
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   neoplastics
shipped in   wet ice
storage temp.   2-8°C
InChI   1S/C35H35NO12/c1-16-12-19(42-4)14-22-20(16)8-9-23(37)27(22)32(40)45-24-13-18-10-11-35(26-15-43-34(41)46-26)25(48-35)7-5-6-21(18)31(24)47-33-28(36-3)30(39)29(38)17(2)44-33/h8-9,12-14,17,21,24-26,28-31,33,36-39H,6,15H2,1-4H3/t17-,21?,24-,25-,26-,28-,29+,30-,31-,33-,35+/m1/s1



Neocarzinostatin from Streptomyces carzinostaticus has been used:
• as a DNA damaging agent to treat human fibroblast GM0637 cells
• as a radiomimetic antibiotic to induce DNA damage
• in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells


100 μg in glass bottle

Biochem/physiol Actions

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G2 cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.

Neocarzinostatin is a radiomimetic antibiotic substance.

Physical form

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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Peer-Reviewed Papers


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