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N9162 Sigma-Aldrich

Neocarzinostatin from Streptomyces carzinostaticus

≥90% (SDS-PAGE), ~0.5 mg/mL

Synonym: Holoneocarzinostatin, NCS, NSC-69856, Zinostatin

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application, Antibiotics by Mechanism of Action,
Quality Level   200
assay   ≥90% (SDS-PAGE)
concentration   ~0.5 mg/mL
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   neoplastics
shipped in   wet ice
storage temp.   2-8°C
InChI   1S/C35H35NO12/c1-16-12-19(42-4)14-22-20(16)8-9-23(37)27(22)32(40)45-24-13-18-10-11-35(26-15-43-34(41)46-26)25(48-35)7-5-6-21(18)31(24)47-33-28(36-3)30(39)29(38)17(2)44-33/h8-9,12-14,17,21,24-26,28-31,33,36-39H,6,15H2,1-4H3/t17-,21?,24-,25-,26-,28-,29+,30-,31-,33-,35+/m1/s1
InChI key   BLXZMHNVKCEIJX-PNKAXBHOSA-N

Description

Application

Neocarzinostatin from Streptomyces carzinostaticus has been used:
• as a DNA damaging agent to treat human fibroblast GM0637 cells
• as a radiomimetic antibiotic to induce DNA damage
• in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells

Packaging

100 μg in glass bottle

Biochem/physiol Actions

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G2 cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.

Neocarzinostatin is a radiomimetic antibiotic substance.

Physical form

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Discover Bioactive Small Molecules for Immunology Research

The immune system is a complex network to detect and defend against non-self pathogenic agents, such as disease-causing viruses, bacteria, and other microorganisms. Various signaling pathways contrib...
Keywords: Antibiotics, Antifungals, Antivirals, Apoptosis, Bioactive small molecules, Cell signaling, Diabetes, Diseases, Immunology, Inflammation

Peer-Reviewed Papers
15

References

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