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  • N9532 - Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

N9532 Sigma

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

clone NOS-E1, ascites fluid

Synonym: Anti-eNOS



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
species reactivity   rat, human, mouse, bovine
application(s)   microarray: suitable
  western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells
clone   NOS-E1, monoclonal
antibody form   ascites fluid
isotype   IgA
mol wt   antigen mol wt 135 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

General description

The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.


Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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