• USA Home
  • N9532 - Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
N9532 Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

clone NOS-E1, ascites fluid

Synonym: Anti-eNOS

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
conjugate   unconjugated
clone   NOS-E1, monoclonal
biological source   mouse
application(s)   microarray: suitable
  western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells
species reactivity   mouse, human, bovine, rat
mol wt   antigen 135 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgA
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P29474
Gene Information   human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)

Description

General description

Monoclonal Anti-Nitric Oxide Synthase, endothelial (eNOS) (mouse IgA isotype) is derived from the NOS-E1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. NOS consists of heme and calmodulin binding sites located within its N-terminal half. It has been localized in many different cell types. Based on molecular mass, subcellular location and Ca2+ dependence, at least three types of NOS have been classified. Type I NOS (150-160 kD protein) is found in neurons and is also called as neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kD protein) is found in macrophages and is also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kD protein) is found in endothelial cells and is also called endothelial NOS (eNOS or ecNOS).

The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

Application

Monoclonal Anti-Nitric Oxide Synthase, Endothelial (eNOS) has been used:
•  for enzyme linked immunosorbent assay (ELISA)
• immunoblot
• immunohistochemistry
• immunofluorescence.

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.

NOS catalyses the oxidation of guanidino nitrogen of arginine. The released nitric oxide mediates vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. NOS is associated with multiple sclerosis and allergic encephalomyelitis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?