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N9879 Sigma-Aldrich

NADase from porcine brain

≥0.007 unit/mg solid

Synonym: DPN Nucleosidase, NAD+ glycohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
form   powder
specific activity   ≥0.007 unit/mg solid
solubility   insoluble
storage temp.   −20°C

Description

General description

NADase is a glycohydrolase that catalyzes ADP-ribose transfer.

Application

NADase from porcine brain has been used in a study to investigate histidine and related compounds resulting from catalyzed ADP-riboslyation. It has also been used in a study to investigate the preparation of arylazide-substituted pyridine adenine dinucleotides for photoaffinity labeling.

Packaging

5 g in poly bottle

Biochem/physiol Actions

Nicotinamide has been shown to inhibit NADase activity.

Unit Definition

One unit will hydrolyze 1.0 μmole of β-NAD to nicotinamide and ADP-ribose per min at pH 7.3 at 37 °C.

Physical form

Acetone-dried powder

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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