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NA0310 Sigma-Aldrich

GenElute HP Plasmid Maxiprep Kit

Green Alternative

sufficient for 25 purifications

Synonym: GenElute HP Plasmid Maxiprep Kit

  •  NACRES NA.52



General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.


GenElute® HP Plasmid Maxiprep Kit has been used in the isolation of plasmids.

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 1.2 mg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• Contains fewer plastic components than other high speed kits, reducing the amount of waste
• No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

Gabitril is a registered trademark of Abbott Laboratories Corp.

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 2924 8(3) / PGIII
Flash Point(F) 
77 °F
Flash Point(C) 
25 °C

Single- & multi-channel pipettes from BRAND
Protocols & Articles


Plasmid DNA Purification Overview

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a ...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Related Content

GenElute™ HP Plasmid Maxiprep Procedure [VIDEO]

The GenElute™ HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate us...
Keywords: Digestions, Peptide synthesis, Polymerase chain reaction, Sequencing, Transfection, transformation

Peer-Reviewed Papers


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