• USA Home
  • NA0410 - GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

NA0410 Sigma-Aldrich

GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

Green Alternative

sufficient for 25 preparations

Synonym: Gen Elute, GenElute Endotoxin-Free Plasmid Kit

  •  NACRES NA.52



General description

The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.


GenElute HP Endotoxin-Free Plasmid Maxiprep Kit has been used in the isolation of plasmid from swine liver and from competent Escherichia coli cells.

Features and Benefits

• Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 35 minutes from pelleted cells to purified plasmid
• Convenient vacuum format


An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Signal word 
Target organs 
Central nervous system
Supplemental Hazard Statements 
UN 2924 8(3) / PGIII
Flash Point(F) 
77.0 °F - closed cup
Flash Point(C) 
25 °C - closed cup
Protocols & Articles


Plasmid DNA Purification Overview

Extraction of macromolecules such as DNA, RNA, and protein is common in molecular biology research. The process of extraction and purification of nucleic acids has evolved from being a complex and la...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

L3287 Escort IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.
93021-001 DNAstable®, Tube kit, for room temp preservation of DNA

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?