• USA Home
  • NA1111 - GenElute Gel Extraction Kit

EMAIL THIS PAGE TO A FRIEND
NA1111 Sigma-Aldrich

GenElute Gel Extraction Kit

sufficient for 70 purifications

Synonym: Rapid purification of linear DNA

Purchase

Description

General description

The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.

Application

GenElute Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.

The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Features and Benefits

• Bind up to 10 μg of DNA
• Recoveries up to 80%
• Up to 3.5 g can be processed per column
• Compatible with both standard and low-melting agarose in TAE or TBE buffer

Principle

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.

The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 1824 8 / PGIII
WGK Germany 
2

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Related Content

GenElute™ Gel Extraction Procedure [VIDEO]

The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels. This kit can also be used to puri...
Keywords: Cloning, Digestions, Melting, Polymerase chain reaction, Purification, Sequencing

Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Add to Cart

Z722390 x-tracta Gel Extraction Tool, pkg of 100 ea

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?