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O0382 Sigma-Aldrich

Anti-Opioid δ Receptor antibody produced in rabbit

whole antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurotransmission, Antibodies to Neurotransmitter Receptors, Neuroscience,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: 1:800 using PLP fixed rat brain sections
  western blot: 1:800 using whole brain homogenate
species reactivity   mouse (predicted), rat
mol wt   antigen ~55 kDa
form   lyophilized powder
shipped in   wet ice
storage temp.   −20°C
antibody form   whole antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P33533
Gene Information   mouse ... Oprd1(18386)
rat ... Oprd1(24613)

Description

General description

Opioid peptides are endogenous neuromodulators that play a major role in nociception by interacting with several membrane receptors. Molecular cloning techniques have characterized the nucleotide sequence of several distinct opioid receptors, including the δ-, κ- and μ-opioid receptors.1 The cloned receptors are highly homologous (65%), differing only at the N- and C-termini and at the extracelluar loops that confer binding specificity.2 All three receptors interact with heterotrimeric G proteins.3
δ-Opioid receptors (DOR) are located postsynaptically on pallidostriatal feedback neurons.4 DORs also modulate nociception presynaptically in the periaqueductal gray where immunolabeling of DOR has been shown to be intracellular and often associated with large dense-core vesicles. 5 Additionally, receptor autoradiographic investigations have localized DORs to the external plexiform layer of the olfactory bulb, the nucleus accumbens, several layers of the cerebral cortex and several nuclei of the amygdala.6

References
1. Goldstein, A. Trends Pharmacol. Sci., 8, 456-459 (1987).
2. Reisine, T., Bell, G.I. Trends Neurosci., 16, 506-510 (1993).
3. Childers, S.R. Life Sci., 48, 1991-2003 (1991).
4. Olive, M.F. et al. J. Neurosci., 17, 7471-7479 (1997).
5. Commons, K.G. et al. J. Comp. Neurol., 430, 200-208 (2001).
6. Quock, R.M. et al. Pharmacol. Rev., 51, 503-532 (1999).

Specificity

The antiserum is specific for the COOH terminal of the opioid δ receptor.

Immunogen

Peptide corresponding to the carboxy-terminal of the opioid δ receptor covalently attached onto a carrier protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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