O1391 Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

Synonym: Solvent Red 27, Sudan Red 5B

  • CAS Number 1320-06-5

  • Empirical Formula (Hill Notation) C26H24N4O

  • Molecular Weight 408.49

  •  EC Number 200-661-7

  •  NACRES NA.47



Related Categories Cell Biology, Hematology and Histology, O, Routine Histology Stains, Stains & Dyes, A to Z,
Quality Level   200
form   liquid
concentration   0.5% in isopropanol
application(s)   hematology: suitable
  histology: suitable
εmax   0.20-0.50 at 510-517 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
InChI   1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-


General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt’s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.


Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.


500 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
Target organs 
Central nervous system
UN1219 - class 3 - PG 2 - Isopropanol, solution
WGK Germany 
Flash Point(F) 
53.6 °F - closed cup
Flash Point(C) 
12.0 °C - closed cup


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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