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O1391 Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

Synonym: Solvent Red 27, Sudan Red 5B

  • CAS Number 1320-06-5

  • Empirical Formula (Hill Notation) C26H24N4O

  • Molecular Weight 408.49

  •  EC Number 200-661-7

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Properties

Related Categories Cell Biology, Hematology and Histology, O, Routine Histology Stains, Stains & Dyes, A to Z,
concentration   0.5% in isopropanol

Description

Application

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.

Packaging

500 mL in glass bottle

General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt’s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1219 - class 3 - PG 2 - Isopropanol, solution
WGK Germany 
2
Flash Point(F) 
53.6 °F
Flash Point(C) 
12 °C

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (c...
Anna Abai, Nick Asbrock and Vi Chu1
1 MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Clinical, Drug discovery, Gene expression, Immunostaining

Mesenchymal Stem Cell FAQs

Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages including bo...
Keywords: Anti-inflammatory agents, Cell disruption, Clinical, Digestions, Diseases, Gene expression, Growth factors, Neurodegenerative Diseases

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods       Human MSC Expansion in Human Platelet Lysate       Human MSC Differentiation       FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials
Aletta Schnitzler, Manjula Aysola, Anjali Verma, Tristan Lawson, Susan Rigby, Megan Pease, Sandhya Punreddy, Julie Murrell, Martha Rook
Stem Cell Bioprocessing Group
Keywords: Cell culture, Cell disruption, Clinical, Culture media, Flow cytometry, Gene expression, Growth factors, Polymerase chain reaction, Stem cell biology

Protocols

Mesenchymal Stem Cell Culture Protocols

Introduction Methods   Isolation of Mesenchymal Stem Cells   Expansion of Human Mesenchymal Stem Cells     Preparation of Coated Plates     Thawing of Mesenchymal Stem Cells     Expansion of Mesenchy...
Keywords: Cell disruption, Clinical, Culture media, Gene expression, Immunocytochemistry, Stem cell biology

Mesenchymal Stem Cell Differentiation

Mesenchymal stem cells (MSCs) are fibroblastoid multipotent adult stem cells with a high capacity for selfrenewal and differentiation. These cells have been isolated from several human tissues, inclu...
Keywords: Evaporation

Peer-Reviewed Papers
15

References

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