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O1516 Sigma-Aldrich

Oil Red O solution

0.5% in propylene glycol

Synonym: Solvent Red 27, Sudan Red 5B

  • CAS Number 1320-06-5

  • Empirical Formula (Hill Notation) C26H24N4O

  • Molecular Weight 408.49

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Properties

Related Categories Cell Biology, Hematology and Histology, O, Routine Histology Stains, Stains & Dyes, A to Z,
concentration   0.5% in propylene glycol

Description

General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt’s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Flash Point(F) 
217.4 °F
Flash Point(C) 
103 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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