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O1516 Sigma-Aldrich

Oil Red O solution

0.5% in propylene glycol

Synonym: Solvent Red 27, Sudan Red 5B

  • CAS Number 1320-06-5

  • Empirical Formula (Hill Notation) C26H24N4O

  • Molecular Weight 408.49

  •  NACRES NA.47

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Properties

Related Categories Cell Biology, Hematology and Histology, O, Routine Histology Stains, Stains & Dyes, A to Z,
Quality Level   200
form   liquid
concentration   0.5% in propylene glycol
application(s)   hematology: suitable
  histology: suitable
λmax   0.40-1.00 at 506-520 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
InChI   1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
InChI key   HJAYEODIXUYVIC-LTFAAIOASA-N

Description

General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt’s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
217.4 °F
Flash Point(C) 
103 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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