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  • O7264 - Anti-Osteopontin antibody produced in rabbit

O7264 Sigma-Aldrich

Anti-Osteopontin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Bone Sialoprotein 1, Anti-Early T Lymphocyte Activation Protein 1 (ETA1), Anti-Secreted Phosphoprotein 1, Anti-Uropontin



Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using human kidney
  western blot: 0.5-1 μg/mL using whole extract of human embryonal kidney 293T cells and a chemiluminescent detection reagent
species reactivity   human
mol wt   antigen ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P10451
Gene Information   human ... SPP1(6696)


General description

Osteopontin (OPN), also termed as bone sialoprotein 1, urinary stone protein, early t lymphocyte activation protein 1 (ETA1), and uropontin, is a phosphorylated acidic arginine-glycine-aspartate (RGD)-containing glycoprotein that binds integrin and CD44 receptors. OPN is the most abundant product of osteoblast and kidney cells, it is also expressed in lung, liver, bladder, pancreas, breast, vascular smooth muscle cells and macrophages.

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.


synthetic peptide corresponding to amino acid residues 249-267 of human osteopontin, con­jugated to KLH.


Anti-Osteopontin antibody produced in rabbit has been used in immunohistochemistry and immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Osteopenia (OPN), functions like chemokine and cytokine. It is implicated in a number of physiologic and pathologic events including angiogenesis, apoptosis, inflammation, wound healing and tumor metastasis. Osteopontin expression is upregulated in cancer of breast, prostate, ovarian, lung, brain tumor and hepatocellular carcinoma.

Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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