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O7635 Sigma-Aldrich

Anti-Osteopontin antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-OPN

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   capture ELISA: 0.2-0.8 μg/mL
  immunohistochemistry: 5-15 μg/mL using mouse thymus tissue sections
  neutralization: 1-3 μg/mL
  western blot: 0.1-0.2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P10923
Gene Information   mouse ... Spp1(20750)

Description

General description

The OPN (osteopontin) gene is mapped to mouse chromosome 5 E5 and codes for secreted phosphoprotein 1 and is mostly expressed in bone tissue.

Immunogen

purified recombinant mouse osteopontin expressed in NSO cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Osteopontin (OPN) serves as a ligand to a number of integrin receptors which are associated with cell motility, chemotaxis, invasion and cell survival. Upregulation of OPN gene is observed in tumors such as colorectal carcinomas, breast cancer and contributes to its pathogenesis. OPN is associated with obesity by mediating adipocyte inflammation and insulin resistance upon macrophage activation. OPN functions as an effector molecule in extracellular signalling and promotes fibrosis and myocyte growth.

Analysis Note

Neutralizes the biological activity of recombinant mouse osteopontin using 293 cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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