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OGS158 Sigma-Aldrich


plasmid vector for molecular cloning

Synonym: cloning vector, expression vector, molecular cloning vector, plasmid vector, plasmid, snapfast vector, vector

  •  NACRES NA.85



Related Categories Cloning and Expression, Molecular Biology, SnapFast Cloning Vectors, SnapFast Vectors for Mammalian Host (untagged)
form   buffered aqueous solution
mol wt   size 8694 bp
Origin of replication   pUC (500 copies)
Peptide cleavage   no cleavage
Promoter   Promoter name: CMV
Promoter type: mammalian
bacteria selection   kanamycin
reporter gene   beta Gal
shipped in   ambient
storage temp.   −20°C


General description

This vector contains a beta galactosidase reporter gene expression cassette under the control of the human Ubiquitin promoter that is designed as an add-on to any of our vectors. The Ub luciferase cassette is flanked by AscI sites but is not designed to be split up. If you would like the luciferase gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus promoter driven vector in the same format if you require an expression vector with lower levels of reporter gene activity.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.


Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.

The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.

Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.


Quick-reference Plasmid Map

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Legal Information

Oxford Genetics is a trademark of Oxford Genetics Ltd

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


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Plasmid Product Nomenclature

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Primers for Plasmid Sequencing

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Restriction Site Positions and Functions

Click on the relevant restriction site to learn more about its function in the SnapFast plasmid system.
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Keywords: Adsorption, Antibiotics, Apoptosis, Cell culture, Cell disruption, Centrifugation, Chromatography, Cloning, Dialysis, Enzyme activity, Flow cytometry, Gene expression, Genetics, Immobilization, Immunoprecipitation, Nucleic acid denaturation, Precipitation, Purification, Transcription, Transfection, Western blot


Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Quick Start Plasmid Vector Guide

SnapFast™ Vectors are supplied in solution as 5µg plasmid DNA in TE buffer, at ambient temperature. If you are not ready to use your DNA right away, store this in the -20 °C for long-term storage, or...
Keywords: Cloning, Genetics, PAGE, Purification, Transfection, transformation

Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being express...
Keywords: AGE, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid annealing, Peptide synthesis, Phase transitions, Phosphorylations, Polymerase chain reaction, Precipitation, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

SnapFast™ Cloning & Expression Vectors FAQs

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Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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Peer-Reviewed Papers


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