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OGS207 Sigma-Aldrich

PSF-OXB20-BETAGAL - BACTERIAL BETA GALACTOSIDASE PLASMID

plasmid vector for molecular cloning

Synonym: cloning vector, expression vector, molecular cloning vector, plasmid vector, plasmid, snapfast vector, vector

Purchase

Properties

Related Categories Cloning and Expression, Molecular Biology, SnapFast Cloning Vectors, SnapFast Vectors for Bacterial Host
form   buffered aqueous solution
mol wt   size 7371 bp
Origin of replication   pUC (500 copies)
Peptide cleavage   no cleavage
Promoter   Promoter name: OXB20
Promoter activity: Constitutive
Promoter type: bacterial
bacteria selection   kanamycin
reporter gene   beta Gal
shipped in   ambient
storage temp.   −20°C

Description

General description

The expression of the beta galactosidase (beta gal) reporter gene under the control of the RecA constitutive bacterial promoter. This plasmid can be used for monitoring reporter gene activity in bacterial cells or colonies using X-Gal which will produce a blue colouration. The reporter gene has been inserted thin the primary multiple cloning site. X-Gal is not membrane permeable and so cells must be lysed prior to detect when screening for blue colonies on an agar plate sufficient cells will naturally lyse on the plate to allow blue colony detection.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Application

Cloning in a gene: This plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.

By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.

Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.

BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.

Sequence

Quick-reference Plasmid Map

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Legal Information

Oxford Genetics is a trademark of Oxford Genetics Ltd

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Genome Compiler

Sigma® Life Science is using our strengths and building on the strengths of others to provide the best tools to synthetic biology researchers. We are happy to participate in Genome Compiler's free, w...
Keywords: Cloning

How to Choose a SnapFast™ Expression Vector

With >1600 vectors to choose from, you have many options when planning your cloning constructs. Finding the perfect expression vector starts with understanding what you need, and what the options are.
Keywords: Cloning, Gene expression, Genetics, Immunoprecipitation, Individual protein Immunoprecipitation, Methylations, Purification, Western blot

Plasmid Product Nomenclature

The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. We currently have hundreds of pre-designed DNA sections that ...
Keywords: Catalysis, Cloning, Digestions, PAGE

Primers for Plasmid Sequencing

We have designed a range of forward and reverse sequencing primers that allow you to sequence any insert that you make into a particular position within any of our SnapFast™ expression vectors. Other...
Keywords: Cloning, Gas chromatography, Gene expression, Melting, Sequencing

Restriction Site Positions and Functions

Click on the relevant restriction site to learn more about its function in the SnapFast plasmid system.
Keywords: Antibiotics, Cloning, Diagnostic, Gene expression, PAGE, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Purification, Recombination, Sequencing

SnapFast™ Cloning & Expression Vectors Selection Charts

With over 1600 vectors to choose from, your options may seem endless. For help choosing the right vector for your research needs, consult the tables below.
Keywords: Cloning, Gene expression, Genetics, Molecular biology, Transcription

What is a DNA Component?

Our plasmids are composed of both a core vector backbone and a series of DNA components. Our plasmid platform is all built around the same core backbone, which means that the DNA components within ea...
Keywords: Adsorption, Antibiotics, Apoptosis, Cell culture, Cell disruption, Centrifugation, Chromatography, Cloning, Dialysis, Enzyme activity, Flow cytometry, Gene expression, Genetics, Immobilization, Immunoprecipitation, Nucleic acid denaturation, Precipitation, Purification, Transcription, Transfection, Western blot

Protocols

Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Quick Start Plasmid Vector Guide

SnapFast™ Vectors are supplied in solution as 5µg plasmid DNA in TE buffer, at ambient temperature. If you are not ready to use your DNA right away, store this in the -20 °C for long-term storage, or...
Keywords: Cloning, Genetics, PAGE, Purification, Transfection, transformation

Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being express...
Keywords: AGE, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid annealing, Peptide synthesis, Phase transitions, Phosphorylations, Polymerase chain reaction, Precipitation, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

SnapFast™ Cloning & Expression Vectors FAQs

Where do I find the sequences and maps of a SnapFast™ plasmid and insert? How can I confirm the orientation of my insert if I use only one insertion site? How have you removed unwanted restriction si...
Keywords: Buffers, Cloning, Diagnostic, Digestions, Gene expression, Genetics, PAGE, Peptide synthesis, Polymerase chain reaction, Sequencing, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Related Content

Synthetic Biology Resources

We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Peer-Reviewed Papers
15

References

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