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OGS553 Sigma-Aldrich

PSF-OXB1 - WEAK STRENGTH BACTERIAL PROMOTER PLASMID

plasmid vector for molecular cloning

Synonym: cloning vector, expression vector, molecular cloning vector, plasmid vector, plasmid, snapfast vector, vector

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Properties

Related Categories Cloning and Expression, Molecular Biology, SnapFast Cloning Vectors, SnapFast Vectors for Bacterial Host
form   buffered aqueous solution
mol wt   size 3859 bp
Origin of replication   pUC (500 copies)
Peptide cleavage   no cleavage
Promoter   Promoter name: OXB1
Promoter activity: Constitutive
Promoter type: bacterial
bacteria selection   kanamycin
reporter gene   none
shipped in   ambient
storage temp.   −20°C

Description

General description

This plasmid contains a weak bacterial promoter for expression in E. coli. It has been derived by modifying the AraBAD (arabinose operon) promoter to remove the AraC repressor site. When tested this promoter was found to be very weak in comparison to most other bacterial promoters including those in our product range. For this reason the promoter is called OXB1 in our collection of bacterial promoters that extend from OXB1 to OXB20 with OXB20 being the strongest. These promoters do not require induction for expression.

Promoter Expression Level: This plasmid contains a weak constitutive E. coli promoter that was derived from the Arabinose operon. It is part of our constitutive bacterial promoter range. This promoter (OXB1) shows the lowest level of expression in the range with OXB20 showing the highest level of expression. They require no inducing agent for expression.

Application

Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.

The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.

Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

Sequence

Quick-reference Plasmid Map

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Legal Information

Oxford Genetics is a trademark of Oxford Genetics Ltd

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Genome Compiler

Sigma® Life Science is using our strengths and building on the strengths of others to provide the best tools to synthetic biology researchers. We are happy to participate in Genome Compiler's free, w...
Keywords: Cloning

How to Choose a SnapFast™ Expression Vector

With >1600 vectors to choose from, you have many options when planning your cloning constructs. Finding the perfect expression vector starts with understanding what you need, and what the options are.
Keywords: Cloning, Gene expression, Genetics, Immunoprecipitation, Individual protein Immunoprecipitation, Methylations, Purification, Western blot

Plasmid Product Nomenclature

The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. We currently have hundreds of pre-designed DNA sections that ...
Keywords: Catalysis, Cloning, Digestions, PAGE

Primers for Plasmid Sequencing

We have designed a range of forward and reverse sequencing primers that allow you to sequence any insert that you make into a particular position within any of our SnapFast™ expression vectors. Other...
Keywords: Cloning, Gas chromatography, Gene expression, Melting, Sequencing

Restriction Site Positions and Functions

Click on the relevant restriction site to learn more about its function in the SnapFast plasmid system.
Keywords: Antibiotics, Cloning, Diagnostic, Gene expression, PAGE, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Purification, Recombination, Sequencing

SnapFast™ Cloning & Expression Vectors Selection Charts

With over 1600 vectors to choose from, your options may seem endless. For help choosing the right vector for your research needs, consult the tables below.
Keywords: Cloning, Gene expression, Genetics, Molecular biology, Transcription

What is a DNA Component?

Our plasmids are composed of both a core vector backbone and a series of DNA components. Our plasmid platform is all built around the same core backbone, which means that the DNA components within ea...
Keywords: Adsorption, Antibiotics, Apoptosis, Cell culture, Cell disruption, Centrifugation, Chromatography, Cloning, Dialysis, Enzyme activity, Flow cytometry, Gene expression, Genetics, Immobilization, Immunoprecipitation, Nucleic acid denaturation, Precipitation, Purification, Transcription, Transfection, Western blot

Protocols

Cloning the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Quick Start Plasmid Vector Guide

SnapFast™ Vectors are supplied in solution as 5µg plasmid DNA in TE buffer, at ambient temperature. If you are not ready to use your DNA right away, store this in the -20 °C for long-term storage, or...
Keywords: Cloning, Genetics, PAGE, Purification, Transfection, transformation

Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being express...
Keywords: AGE, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid annealing, Peptide synthesis, Phase transitions, Phosphorylations, Polymerase chain reaction, Precipitation, transformation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

SnapFast™ Cloning & Expression Vectors FAQs

Where do I find the sequences and maps of a SnapFast™ plasmid and insert? How can I confirm the orientation of my insert if I use only one insertion site? How have you removed unwanted restriction si...
Keywords: Buffers, Cloning, Diagnostic, Digestions, Gene expression, Genetics, PAGE, Peptide synthesis, Polymerase chain reaction, Sequencing, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Related Content

Synthetic Biology Resources

We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Peer-Reviewed Papers
15

References

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