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  • P0058 - Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit

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P0058 Sigma-Aldrich

Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-1-Cys peroxiredoxin, Anti-Acidic calcium-independent phospholipase A2, Anti-Antioxidant protein 2, Anti-Non-selenium glutathione peroxidase, AOP2, Anti-PRDX6

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 2-5 μg using whole extract of mouse brain
  western blot: 0.5-1.0 μg/mL using whole extract of human HeLa cells.
  western blot: 1-2 μg/mL using whole extract of rat brain
species reactivity   mouse, human, rat
mol wt   antigen ~25 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P30041
Gene Information   human ... PRDX6(9588)
mouse ... Prdx6(11758)
rat ... Prdx6(94167)

Description

General description

PRDX6 (peroxiredoxin 6) is a mammalian peroxidase belonging to the 1-Cys Prdx family, meaning it has 1-Cys at position 47 (Cys47). This protein is localized to cytoplasm, lysosomes, mitochondria, endoplasmic reticulum (ER) and plasma membrane. It is a homodimeric protein which is especially observed in mammalian lungs.

Application

Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit has been used for Western Blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

PRDX6 (peroxiredoxin 6) has both peroxidase and phospholipase A2 (PLA2) activities and thus, is a bifunctional protein. In liver, as a peroxidase it confers protection against damage from ROS (reactive oxygen species) during ischemia-reperfusion injury. As a PLA2 it is involved in lung surfactant metabolism. It is also essential for normal nicotinamide adenine dinucleotide phosphate-oxidase activity. In the livers of donors after brain death (DBD), this protein suppresses ischemia- and hypoxia-induced liver damage, and its expression is controlled by NF-κB (nuclear factor). In human and mouse neuronal cells, the delivery of PRDX6 protein leads to delay or suppression in oxidative stress-induced neuronal cell death.

Peroxiredoxin 6 contains a single redox-active cysteine and uses glutathione to catalyze the reduction of H2O2 and other organic peroxides. Overexpression of peroxiredoxin 6 in cells protects them against oxidative damage, whereas knockdown of this enzyme results in oxidative stress and apoptosis. The phospholipase A2 activity plays an important role in surfactant homeostasis. Peroxiredoxin 6 is a major antioxidant enzyme which functions in antioxidant defense and lung phospholipid metabolism.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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P0050 Anti-Peroxiredoxin 6 (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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