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P0065 Sigma-Aldrich

Phospholipase D from Streptomyces chromofuscus

≥50,000 units/mL, buffered aqueous glycerol solution

Synonym: Lecithinase D, Phosphatidylcholine phosphatidohydrolase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
form   buffered aqueous glycerol solution
mol wt   mol wt ~60 kDa
shipped in   dry ice
storage temp.   −70°C

Description

Biochem/physiol Actions

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

Unit Definition

One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 37 °C.

Physical form

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

Application

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

General description

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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