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  • P0085 - Anti-Podoplanin antibody, RAT monoclonal

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P0085 Sigma-Aldrich

Anti-Podoplanin antibody, RAT monoclonal

clone NZ-1.2, purified from hybridoma cell culture

Synonym: Anti-AGGRUS, Anti-GP36, glycoprotein, 36-KD, Anti-GP40, Anti-Gp38, Anti-OTS8, Anti-T1A-2, Anti-T1A

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Other Cellular and Organelle Proteins,
conjugate   unconjugated
clone   NZ-1.2, monoclonal
biological source   rat
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 4-8 μg/mL using U87 total cell extract
species reactivity   human
mol wt   antigen ~36 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   Q86YL7
Gene Information   human ... PDPN(10630)

Description

General description

Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stimulating (PLAG) domain of podoplanin. Podoplanin (Aggrus) is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has been described to be present in normal, inflammatory and neoplastic lymphatic capillary endothelium.

Immunogen

platelet-aggregation-stimulating (PLAG) domain of human podoplanin.

Application

Anti-Podoplanin antibody, Mouse monoclonal has been used in:
• immunoblotting
• immunoprecipitation
• flow cytometry
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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