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P0085 Sigma

Monoclonal Anti-Podoplanin antibody produced in rat

~1.5 mg/mL, clone NZ-1.2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-AGGRUS, Anti-GP36, glycoprotein, 36-KD, Anti-GP40, Anti-Gp38, Anti-OTS8, Anti-T1A-2, Anti-T1A

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Other Cellular and Organelle Proteins,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 4-8 μg/mL using U87 total cell extract
clone   NZ-1.2, monoclonal
concentration   ~1.5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
mol wt   antigen mol wt ~36 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PDPN(10630)
biological source   rat
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

platelet-aggregation-stimulating (PLAG) domain of human podoplanin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Amplification, Buffers, Diagnostic, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Therapeutic uses, Western blot

Peer-Reviewed Papers
15

References

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P1995 Anti-Podoplanin (HG-19) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
P5374 Anti-Podoplanin antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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