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  • P0104 - Monoclonal Anti-PIASy antibody produced in mouse

P0104 Sigma

Monoclonal Anti-PIASy antibody produced in mouse

~1.5 mg/mL, clone PIA4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-PIAS4 (protein inhibitor of activated STAT, 4), Anti-Piasg



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to E1,E2,E3 Ligase, Antibodies to Protein Degradation,
species reactivity   human
application(s)   immunocytochemistry: suitable
  western blot: 1-2 μg/mL using total cell extract of HEK-293T cells transfected with human PIASy
clone   PIA4, monoclonal
concentration   ~1.5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PIAS4(51588)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to amino acids 491-504 of human PIASy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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