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  • P0123 - Anti-Paraoxonase 1 (PON1) antibody produced in rabbit

P0123 Sigma-Aldrich

Anti-Paraoxonase 1 (PON1) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Arylesterase, Anti-Coronary Artey Disease, Susceptibility to, Anti-Coronary Spasms, Susceptibility to, Anti-Esterase A (ESA), Anti-Organophosphate Poisoning, Sensitivity to, Anti-PON1, Anti-Parathion Poisoning, Sensitivity to



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 5-10 μg/mL using human HT-29 cells
  western blot: 0.5-1 μg/mL using whole extract of human colorectal adenocarcinoma HT-29 cells
species reactivity   mouse (predicted), human, rat (predicted)
mol wt   antigen ~40 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P27169
Gene Information   human ... PON1(5444)
mouse ... Pon1(18979)
rat ... Pon1(84024)


General description

Paraoxonase-1 (PON1) is a member of serum paraoxonases family, consisting of PON1, PON2, and PON3. Human PONs map to the long arm of chromosome 7. PON1 is polymorphic in human populations, and different individuals express widely different levels of this enzyme. PON1 and PON3 are expressed in the liver and secreted into the blood where they are associated with the high-density lipoprotein (HDL) particle.


synthetic peptide corresponding to amino acids 298-309 of human PON1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in mouse and two amino acids in rat. The peptide sequence differs by one amino acid in human PON2 and two amino acids in human PON3.


Anti-Paraoxonase 1 (PON1) antibody produced in rabbit has been used in immunoblotting, and immunofluorescence .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Paraoxonase-1 (PON1) protects lipids from oxidation in lipoproteins, macrophages, and erythrocytes. PON1 may confer protection against coronary artery disease by destroying proinflammatory oxidized lipids present in oxidized low-density lipoproteins (LDLs). PON1 plays a key role in the detoxification of organophosphate insecticides such as parathion, chlorpyrifos and diazinon and nerve gases such as soman and sarin.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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