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P0520 Sigma

Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart

lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
mol wt   mol wt ~60 kDa
composition   Protein, ~75%
foreign activity   5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 unit/mg protein
  5′-nucleotidase <0.01 unit/mg protein
  inorganic pyrophosphatase <0.5 unit/mg protein
storage temp.   −20°C
Gene Information   cow ... PDE1A(281969)


Analysis Note

Protein determined by Warburg-Christian unless specified otherwise.


Package size based on activated units

Preparation Note

Crude preparation which has been stripped of the protein activator, calmodulin.


Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. If stored at 4 °C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.


Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.

For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.

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Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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