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P1374 Sigma

Anti-Pro-BDNF antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym: Anti-pro-Brain-derived Neurotrophic Factor

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Neurotrophin,
species reactivity   rat, mouse, human
application(s)   western blot: 1:200 using rat glioma C6 and human neuroblastoma SH-SY5Y cell lines
  western blot: 1:400 using recombinant mouse proBDNF
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... BDNF(627)
mouse ... Bdnf(12064)
rat ... Bdnf(24225)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

peptide corresponding to amino acid residues 72-88 of human BDNF (precursor). This sequence has 16/17 residues identical in mouse and rat.

General description

Does not cross react with pro-NGF, pro-NT-3, or mature BDNF.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, with 1% bovine serum albumin and ≤0.1% sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) belongs to a family of secreted proteins called neurotrophins. Neurotrophins are synthesized as preproneurotrophin precursors and further processed to proneurotrophins and subsequently to neurotrophins. BDNF is expressed in the developing and the mature brain. The influence of BDNF is mediated by its binding to TrkB receptor and sometimes the receptor p75NTR. The downstream pathways promoted by BDNF include PI3K, MAPK and JAK/STAT. The most important role of BDNF is the regulation of synaptic transmission and plasticity, protein synthesis and phosphorylation of local proteins.

Price and Availability

Safety & Documentation

Safety Information

Hazard statements  H412
Precautionary statements  P273
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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