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P1526 Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate

  • CAS Number 615-28-1

  • Linear Formula C6H4(NH2)2·2HCl

  • Molecular Weight 181.06

  •  Beilstein/REAXYS Number 3912045

  •  EC Number 210-418-7

  •  MDL number MFCD00012966

  •  PubChem Substance ID 24898213

  •  NACRES NA.83

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates,
Quality Level   300
color   white to off-white
mp   258 °C (dec.) (lit.)
storage temp.   −20°C
SMILES string   Cl[H].Cl[H].Nc1ccccc1N
InChI   1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key   RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

Application

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Packaging

1 kg in glass bottle

10, 25, 50, 100 g in glass bottle

Caution

May darken in storage.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
WGK 1
RTECS 
ST0175000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

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Peer-Reviewed Papers
15

References

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