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P1585 Sigma

PMA

for use in molecular biology applications, ≥99% (HPLC)

Synonym: 12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, Phorbol 12-myristate 13-acetate, TPA

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Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
InChI Key   PHEDXBVPIONUQT-RGYGYFBISA-N
assay   ≥99% (HPLC)
form   film
storage condition   protect from light
solubility   DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.)
  ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Potent tumor promoter; activates protein kinase C in vivo and in vitro.

Caution

Photosensitive, protect from light.

Application

Suitable for
• molecular biology studies as standard
• analytical studies of tumor promoters
• promotion of cell growth

General description

PMA (Phorbol 12-myristate 13-acetate) is an activator of protein kinase C (PKC) and a known tumor promoting agent. PMA also exhibits clastogenic (chromosome-breaking) activity.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QH4377000

Frequently Asked Questions

How can I dissolve Phorbol 12-myristate 13-acetate, Product P1585?
Phorbol 12-myristate 13-acetate (PMA) is soluble in DMSO at a 20 mM concentration.  It is also soluble in ethanol. 
What is the stability of stock solutions of Phorbol 12-myristate 13-acetate, Product P1585, and how should I store them?
Stock solutions in DMSO can be stored in the dark as aliquots at -20 deg. C for at least six months.  This, along with other useful information, can be found on this datasheet:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/p1585dat.Par.0001.File.tmp/p1585dat.pdf
How much Phorbol 12-myristate 13-acetate, Product P1585, product should I use?
A concentration of 50 ng/ml was used on Jurkat cells.  This, along with other useful information, can be found on this data sheet:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/p1585dat.Par.0001.File.tmp/p1585dat.pdf
There isn't any powder in the Phorbol 12-myristate 13-acetate, Product P1585, vial?
The product is usually a clear film on the walls of the vial and may not be easily visible.
What is the storage temperature for Phorbol 12-myristate 13-acetate, Product P1585?
Phorbol 12-myristate 13-acetate should be stored at -20 deg. C.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/4/p1585dat.Par.0001.File.tmp/p1585dat.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How is Product P1585, Phorbol-12-myristate-13-acetate, packaged?
P1585-1MG, P1585-10MG and P1585-25MG come to us pre-packaged by our supplier; all three sizes are packaged the same way. The packaging consists of an amber serum vial with a septum and an aluminum crimp cap. The septum has a PTFE (Teflon) liner, so the material inside the vial could only come in contact with glass and Teflon.
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Protocols & Articles

Protocols

Phorbol 12-myristate 13-acetate Molecular Biology Reagent Protocol

T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule. This binding event triggers the rapid hydrolysis of inositol phospholipids to di...
Keywords: Addition reactions, Centrifugation, Enzyme-linked immunosorbent assay, Ligands

Peer-Reviewed Papers
15

References

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