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P1598 Sigma-Aldrich

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

  •  NACRES NA.25

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, General Reagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for RNAi,
Quality Level   100
grade   for molecular biology
form   liquid
usage   80 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   2-8°C

Description

General description

Sigma’s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Components

Sigma’s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Introduction to Markers and Ladders

We offer a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, PAGE, Polymerase chain reaction, Proteomics

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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