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P1787 Sigma-Aldrich

Protein Kinase CβI isozyme human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Synonym: Ca2+-activated phospholipid-dependent serine-threonine kinase, PKCβI

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Active Kinases, Application Index, Biochemicals and Reagents,
Quality Level   200
recombinant   expressed in baculovirus infected insect cells
assay   ≥95% (SDS-PAGE)
form   buffered aqueous glycerol solution
specific activity   ≥700 units/mg protein
mol wt   apparent mol wt 79-80 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKCB(5579)

Description

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Unit Definition

One unit will transfer 1 nmol of phosphate to histone H1 in 1 min at pH 7.4 at 30 °C.

Physical form

Solution in 20 mM HEPES, pH 7.4; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 100 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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Peer-Reviewed Papers
15

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