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P1903 Sigma-Aldrich

Pyruvate Kinase from Bacillus stearothermophilus

Type VIII, lyophilized powder, 100-300 units/mg protein

Synonym: ATP:pyruvate 2-O-phosphotransferase, PK

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Description

Application

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

Packaging

1000 units in glass bottle

Biochem/physiol Actions

Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.

Unit Definition

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.2 at 30 °C.

Physical form

Lyophilized powder containing Tris buffer salts, pH 8.5

Analysis Note

Protein determined by biuret

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

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