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  • P2092 - Monoclonal Anti-p27Kip1 antibody produced in mouse

P2092 Sigma

Monoclonal Anti-p27Kip1 antibody produced in mouse

clone DCS-72, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   rat, canine, mouse, human, monkey
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 1:200 using extract of cultured mouse fibroblast line NIH3T3
clone   DCS-72, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 27 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDKN1B(1027)
mouse ... Cdkn1b(12576)
rat ... Cdkn1b(83571)
biological source   mouse
conjugate   unconjugated



recombinant rodent p27Kip1 protein.


Using immunoblotting, some preparations may exhibit higher and lower molecular weight bands. which may represent a polyubiquitinated species and breakdown products of p27Kip1.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

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RIDADR  NONH for all modes of transport
WGK Germany  2
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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