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P2287 Sigma

TWEEN® 20

viscous liquid, cell culture tested

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate, Polysorbate 20

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Biological Detergents, Cell Biology, Cell Culture,
Quality Level   PREMIUM
description   non-ionic
form   viscous liquid
mol wt   mol wt ~1228
composition   lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
  1.1 g/cm3 at 25 °C(lit.)
HLB   16.7
suitability   cell culture tested

Description

Application

Non-ionic detergent

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Packaging

100, 500 mL in poly bottle

4 L in poly bottle

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Cell Culture Guide

Wired Weeks Savings
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Protocols

A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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