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P2318 Sigma-Aldrich

Anti-Pseudomonas Exotoxin A antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, PS-PS, Primary Antibodies More...
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:20,000
  indirect ELISA: 1:250,000
species reactivity   (Pseudomonas aeruginosa)
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
antibody product type   primary antibodies
contains   15 mM sodium azide

Description

General description

Reacts against Pseudomonas exotoxin A, but shows no reaction against Staphylococcal enterotoxin A, Cholera toxin, and Staphylococcal enterotoxin B. The product has not been tested for neutralization potency against active Pseudomonas exotoxin A.

Immunogen

exotoxin A from Pseudomonas aeruginosa.

Application

Anti-Pseudomonas Exotoxin A antibody has been used in in vitro serum stability of fusion toxins and binding of targeted toxins.

Anti-Pseudomonas exotoxin A antibody produced in rabbit has been used:
• in the in vitro analysis of the stability of fusion toxins in serum
• to construct an epidermal growth factor receptor (EGFR)-targeted toxin to study its toxicity on Swiss mouse embryo NIH-3T3 cells transfected with human EGFR
• in the determination of the in vivo half-life of immunotoxins in serum

Physical form

This product is supplied as a liquid containing 15 mM sodium azide as preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Pseudomonas exotoxin A is secreted by Pseudomonas aeruginosa and has potent cytotoxic effects. Exotoxin A enters the cells by receptor-mediated endocytosis, translocates to the cytoplasm and inhibits protein synthesis by ADP-ribosylating factor 2. Chimeric exotoxin A has applications in gene treatment of various diseases and probable treatment for cancer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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