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P2419 Sigma-Aldrich

Monoclonal Anti-Peroxidase antibody produced in mouse

clone P6-38, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, PD-PG, Plant Biology,
conjugate   unconjugated
clone   P6-38, monoclonal
biological source   mouse
application(s)   indirect ELISA: 1:5,000 using mouse primary antibody, bridging antibody and horseradish peroxidase
species reactivity   horseradish
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide

Description

General description

Monoclonal anti-peroxidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

horseradish peroxidase

Application

Monoclonal Anti-Peroxidase antibody may be used for:
• immunocytochemistry
• indirect ELISA (1:5,000)
• immunohistochemistry
• immunoblotting

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Peroxidase is often used as an enzyme label for antibodies during analyses of target antigens. Peroxidases aids in substrate conversion indicated by the formation of a coloured product. In an alternative procedure, an anti-enzyme antibody can be used to bridge a primary antibody and enzyme complex for the study of target proteins. Anti-peroxidase antibodies are also used to prepare peroxidase-anti-peroxidase (PAP) soluble complexes. Monoclonal Anti-Peroxidase antibody can be used for signal amplification in a wide range of immunological techniques. The product is specific for horseradish peroxidase. Such immunoassays are useful tools to detect antigens, toxins, bacteria and viruses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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