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  • P2860 - Monoclonal Anti-PSF antibody produced in mouse

P2860 Sigma-Aldrich

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies to Nuclear Proteins,
conjugate   unconjugated
clone   B92, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  microarray: suitable
  radioimmunoassay: suitable using cells
  western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
species reactivity   mouse, human
form   buffered aqueous solution
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~2 mg/mL


General description

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.


lysate of mouse bone marrow-derived stromal cell line.


Monoclonal Anti-PSF antibody produced in mouse has been used in:
• immunofluorescence
• immunohistochemistry
• immunoprecipitation

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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