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P2860 Sigma-Aldrich

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies to Nuclear Proteins,
conjugate   unconjugated
clone   B92, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  microarray: suitable
  radioimmunoassay: suitable using cells
  western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
species reactivity   mouse, human
form   buffered aqueous solution
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~2 mg/mL

Description

General description

PSF (polypyrimidine tract-binding protein-associated splicing factor) is an ubiquitous nuclear matrix protein which plays a pivotal role in mammalian spliceosome. The PTB/PSF complex formation is also necessary for pre-mRNA splicing. Monoclonal anti-PSF antibody can be used as a primary antibody in immunofluorescence staining. This antibody is also useful in affinity chromatography, immunoblotting and immunocytochemistry. Monoclonal anti-PSF antibody reacts specifically with human and mouse PSF.

PSF (polypyrimidine tract-binding protein-associated splicing factor) is an ubiquitous nuclear matrix protein which plays a pivotal role in mammalian spliceosome. The PTB/PSF complex formation is also necessary for pre-mRNA splicing.

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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