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  • P2992 - Monoclonal Anti-p59fyn antibody produced in mouse

P2992 Sigma

Monoclonal Anti-p59fyn antibody produced in mouse

~1 mg/mL, clone 1S, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Fyn,
species reactivity   human, rat, mouse
application(s)   capture ELISA: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-5 μg/mL using human lymphoma tissue
  immunoprecipitation (IP): suitable
  western blot: suitable
clone   1S, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... FYN(2534)
mouse ... Fyn(14360)
rat ... Fyn(25150)
biological source   mouse
conjugate   unconjugated



amino acids 1-206 from recombinant murine p59fyn

General description

The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Two non-receptors PTK that belong to Src family, Fyn and Lck are vital for T-cell development and function. Both Fyn and Lck have unique domains that undergo post-translational modifications such as palmitoylation, myristoylation and acetylation od specific cysteine residues that are critical for T cell signalling. Fyn is generally associated with intracellular structures such as mitotic spindles. Both Fyn and Lck are closely associated in T cell differentiation, expansion and mediating TCR-mediated survival signals in naïve T cells. Both the kinases have partially overlapping functions and have the ability to compensate if either kinase is deficient.
Monoclonal Anti- p59fyn specifically recognizes human, mouse and rat Fyn protein (~59 kDa).

Physical form

Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide.


Monoclonal Anti- p59fyn may be used for detection by immunohistochemistry of formalin-fixed, paraffin-embedded human lymphoid tissue sections at a working concentration of 1-5 μg/mL. The recommended concentration for immunoprecipitation is 2μg/mg of lysate. The antibody is also suitable for immunoblotting and ELISA.

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